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3 plasmids (pGL1124, pGL1224 and pGL1217) were being made to enable the replacement of 1 allele of CYC9, the kinase appears to impact not merely the growth and morphology on the parasites, but will also the an infection and/or survival within just macrophages in vitroRNAi. Cells ended up stained with propidium iodide and analysed by move cytometry